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Neural stem/progenitor cells (NSPCs) were cultured following well-established standard protocols in an enriched medium containing 20 ng/ml bFGF and 20 ng/ml EGF. Firstly, a CCK8 assay indicated that NSPCs grown on Poly-L-ornithine (PO) exhibited higher absorbance at 450 nm on both the 7th and 14th days compared to those grown on Poly-L-lysine (PLL) or Fibronectin (FN), suggesting that NSPCs proliferated more robustly on PO. Secondly, Ki-67 staining results showed that the percentage of Ki-67 and Nestin co-labeling was significantly higher in NSPCs cultured on PO than in those cultured on PLL or FN on both the 7th and 14th days. Thirdly, Western blot data revealed that the expression levels of Nestin, a marker preferentially expressed in NSPCs, were significantly higher in NSPCs grown on PO compared to those grown on PLL or FN on both the 7th and 14th days. These data indicate that PO has a superior capability to promote NSPC proliferation compared to PLL or FN. Check more details about Poly-L-ornithine solution.