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Neural stem/progenitor cells (NSPCs) were cultured following well-established standard protocols in an enriched medium containing 20 ng/ml bFGF and 20 ng/ml EGF. Firstly, a CCK8 assay indicated that NSPCs grown on Poly-L-ornithine (PO) exhibited higher absorbance at 450 nm on both the 7th and 14th days compared to those grown on Poly-L-lysine (PLL) or Fibronectin (FN), suggesting that NSPCs proliferated more robustly on PO. Secondly, Ki-67 staining results showed that the percentage of Ki-67 and Nestin co-labeling was significantly higher in NSPCs cultured on PO than in those cultured on PLL or FN on both the 7th and 14th days. Thirdly, Western blot data revealed that the expression levels of Nestin, a marker preferentially expressed in NSPCs, were significantly higher in NSPCs grown on PO compared to those grown on PLL or FN on both the 7th and 14th days. These data indicate that PO has a superior capability to promote NSPC proliferation compared to PLL or FN. Check more details about Poly-L-ornithine solution.

Targeted transcriptional analysis was employed to evaluate the effects of Disperse Yellow 7 (DY7) on cellular stress, thyroid hormone, and reproductive systems. In experiments with DY7-contaminated water, no changes in gene expression were detected. Similarly, animals exposed to sediments containing DY7 did not exhibit changes in mRNA levels of trbeta, dio2, hsp30, and eralpha at any tested concentration. However, after exposure to the highest treatment concentration, hsp70 and hsp90 mRNA levels increased by 2.5-fold and 2.4-fold respectively (p < 0.01). Additionally, a significant 1.8-fold increase in hsp90 mRNA levels was observed at a treatment concentration of 2 µg/g (p = 0.016). Exposure to DY7 also impacted androgen-related genes ar and srd5α2. The expression of ar mRNA was reduced by approximately 2-fold in treated groups, but this reduction was statistically significant only in the 14 and 209 µg/g treatments (p = 0.029). Moreover, at the highest DY7 concentration (209 µg/g), srd5α2 mRNA was induced 2.6-fold (p = 0.024).

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หากคุณคือหนึ่งในนักเดิมพันที่เบื่อกับระบบล็อกยูส เล่นยังไงก็ไม่แตก ถึงเวลาที่คุณต้องรู้จักกับ เบทฟิก285 เว็บตรงคุณภาพที่กล้ารับประกันความยุติธรรม ทุกเกมที่เปิดให้เล่นเป็นลิขสิทธิ์แท้จากค่ายหลักไม่ผ่านเอเย่นต์ มีเกมให้เลือกมากกว่า 1,000 รายการ ไม่ว่าจะเป็น PG / JILI / Joker หรือ Pragmatic ทุกค่ายถูกรวมไว้ในระบบที่ใช้งานง่าย จัดเรียงเกมแบบไม่งง กดเข้าเล่นไว โหลดเร็วไม่มีสะดุด